Our laboratory has two major research interests. One of them is in the field of stem cell biology, wherein we are establishing novel embryonic stem cells (ESC) as well as induced pluripotent stem (iPS) cells from large animal species, for regenerative medicine as well as genetic engineering (transgenics) applications. A second distinct research interest is in the field of reproduction, where we employ human (h) ESC as well as mouse models for investigating human placental pathologies such as pre-eclampsia.
Our laboratory is at the forefront of establishing and characterizing novel ESC and iPS cell lines from domestic pig, which are similar to either mouse (naïve) or human (primed) ESC. Our primary goal is to dissect the molecular pathways that establish and sustain these distinct pluripotent states in this model species, and secondarily to improve the overall efficacy of this process. Ultimately, our objective is to utilize the established cells for cellular therapies (Regenerative medicine) and as tools for genetic manipulation and the establishment of live offspring (Transgenics).
A second interest in the laboratory is to develop in vitro as well as in vivo models for investigating human pregnancy disorders, specifically pre-eclampsia. Pre-eclampsia is one of the major contributors of pre-term births and neonatal mortality worldwide. It is a serious condition with long term consequences to both the mother and the offspring. For the in vitro studies, we are using a hESC based model. HESC can undergo directed differentiation into trophoblast/placental cell types upon exposure to the growth factor BMP4, making it an ideal system for studying early trophoblast specification events. For in vivo studies, we make use of the placental specific transgenesis approach in mice, where the placental tissue is specifically targeted by lentivirus with the gene of interest.
In summary, our laboratory extensively employs stem cells and advanced molecular biology tools for biomedical research. For more details regarding our research or for potential employment, please direct your queries to the investigator:email@example.com
In order of significance; * Corresponding author, ** Equal contribution
Telugu, B.P. *, Ezashi, T., Alexenko, A.P., Spate, L., Prather, R.S., Roberts, R.M. (2011) LIF- Dependent, Pluripotent Stem Cells Established from Inner Cell Mass of Porcine Embryos (Sus scrofa). Journal of Biological Chemistry PMID:21705331.
Telugu, B.P.*, Ezashi, T., Roberts R.M. (2010). Porcine Induced Pluripotent Stem Cells Analogous to naive and primed Embryonic Stem Cells of the Mouse. Intl J Dev Biol 54(11-12):1703-11. PMID:21305472.
Telugu, B.P.*, Ezashi,T., Roberts R.M. (2010). The Promise of Stem Cell Research in Pigs and Other Ungulate Species. Stem Cell Rev. 6(1):31-41. PMID:19949895.
Ezashi, T., Telugu, B.P., Alexenko, A.P., Sachdev, S.C., Sinha, S., Roberts, R.M. (2009). Derivation of Induced Pluripotent Stem Cells from Pig Somatic Cells. PNAS 106(27):10993-8. PMID: 19541600.
Ezashi, T., Matsuyama, H., Telugu, B.P., Roberts, R.M. (2011). Generation of colonies of induced trophoblast cells during standard reprogramming of porcine fibroblasts to induced pluripotent stem (iPS) cells. Biology of Reproduction. PMID:21734265.
Zhou, L., Wang, W., Ezashi,T., Telugu, B.P., Kaplan, H.J., Dean, D.C. (2011). Differentiation of Swine iPSC into Rod Photoreceptors and Their Integration into the Retina. Stem Cells 29(6):972-80. PMID:21491544.
>Telugu, B.P. et al., Bovine Genome Sequencing and Analysis Consortium (2009). The Genome Sequence of Taurine Cattle: a Window to Ruminant Biology and Evolution. Science 324: 522-528. PMID:19390049.
Roberts, R.M., Telugu, B.P., Ezashi, T. (2009). Induced Pluripotent Stem Cells from Swine (Sus scrofa): Why they may Prove to be Important. Cell Cycle 8(19):3078-81. PMID:19738434.
Telugu, B.P., Walker, A.M., Green, J.A. (2009). Characterization of the Bovine Pregnancy-Associated Glycoprotein Gene Family- Analysis of Gene Sequences, Regulatory Regions within the Promoter and Expression of Selected Genes. BMC Genomics 10:185. PMID:19393060.
Telugu, B.P., Palmiere, M., Van Doren, S., Green, J.A. (2010). An examination of the Proteolytic Activity for Bovine Pregnancy-Associated Glycoprotein 2 and 12. Biol chem. 391(2-3):259-70. PMID:20030586.
Telugu, B.P., Spate, L., Prather, R., Green, J.A. (2009) Acid Peptidase Activity Released from In Vitro Produced Porcine Embryos: a Candidate Marker to Predict Developmental Competence. Mol. Reprod. Dev. 76:417-28. PMID:18937336- Featured Article of the issue.
Telugu, B.P., Green, J.A. (2008). Characterization of the Peptidase Activity of Recombinant Porcine Pregnancy-Associated Glycoprotein-2. J.Biochem. 144: 725-32. PMID:18835827.
Green, J.A., Parks, T.E., Avalle, M.P., Telugu, B.P., McLean, A.L., Peterson, A.J., McMillan, W., Mathialagan, N., Hook, R.R., Xie, S., Roberts, R.M. (2005). The Establishment of an ELISA for the Detection of Pregnancy-Associated Glycoproteins (PAGs) in the Serum of Pregnant Cows and Heifers. Theriogenology 63: 1481-503. PMID:15725453.
Telugu, B.P., Wooding FBP, Roberts RM, Green JA (2005). Pregnancy-associated glycoproteins: A complex family of trophoblast genes expressed by the ungulate placenta. In: Maternal Recognition of Pregnancy in the mare III. P41-43. Strout TAE & Wade JF, Eds. Havemeyer Foundation Monograph, Series No. 16. R and W communications, UK. [Proceedings held 13-16 November 2004].
Telugu, B.P., Green, J.A. (2007) Comparative Placentation. In: Comparative Reproductive Biology. P.271-320. Constantinescu, G.M. & Schatten, H, Eds. Blackwell publishing, Iowa City.
Ezashi T., Telugu, B.P., Roberts, R.M (2010). Generation and Characterization of Induced Pluripotent Stem Cells from Pig.P.413-426. In: Stem Cells & Regenerative Medicine. Krishnarao Appasani (Ed). Humana Press, Springer Science+Business Media, LLC, New York.
Ezashi, T., Telugu, B.P., Roberts, R.M. (2011). Dynamic Changes in Gene Expression During Early Trophoblast Differentiation in Human Embryonic Stem Cells Treated with BMP4. In: Embryonic Stem Cells: The Hormonal Regulation of Pluripotency and Embryogenesis. P235-250. Craig S. Atwood (Ed). Intech Publishing, Croatia.
Telugu, B.P., Green, J.A. (2011). Pepsin F. In: Handbook of Proteolytic Enzymes. Rawlings, N.D., & Salvesen, G, Eds. Elsevier publishing, Oxford, UK [submitted].
Telugu, B.P., Green, J.A. (2011). Pregnancy-Associated Glycoproteins. In: Handbook of Proteolytic Enzymes. Rawlings,N.D., & Salvesen, G, Eds. Elsevier publishing, Oxford, UK [submitted].
Ezashi, T., Telugu, B.P., Roberts, R.M. Model Systems for Studying Trophoblast Differentiation from Human Pluripotent Stem Cells. [submitted to Cell & Tissue Research].
Schlitt, J.S.**, Telugu, B.P.**, Ezashi,T., Roberts, R.M., Schulz, L.C. Extravillous trophoblast models from Human Embryonic Stem Cells.