Phone: 301-405-2516
Email: TEPorter@umd.edu
Awards and Honors:
- Gamma Sigma Delta Research Award, May, 2003.
- College of Agriculture and Natural Resources Alumni Association Faculty
Research Award, April,
2001.
- Research highlighted on the cover of Endocrinology, July, 2000.
- Recipient of the College of Agriculture and Natural Resources 2000
Junior Faculty Excellence Award.
- Research
highlighted in an Endocrinology editorial, November, 1997.
- Poultry Science Association Research Award, 1996.
Our research interests center on the neuroendocrine regulation of growth and metabolism. One major focus in our lab is the cellular differentiation of the anterior pituitary gland during embryonic development. Particular emphasis has been placed on the last two cell types to differentiate, the somatotrophs and lactotrophs, which secrete growth hormone and prolactin, respectively. Cell and molecular biology approaches are used to assess gene expression and hormone secretion from individual pituitary cells. These measurements of hormone production are made at specific stages of development or following experimental treatment of developing embryos or pituitary cells in culture. Our working model includes regulation by hormones from other endocrine glands as well as nuclear transcription factors and signal transduction cascades. Long-range goals of this project include defining the intracellular and extracellular regulation of pituitary cell differentiation during embryonic development. A second major focus in our lab is the study of global gene expression in the neuroendocrine system. High density cDNA microarrays, containing copies of more than 18,000 expressed genes have been developed to analyze levels of mRNA in chicken tissues. Rather than studying each gene, one at a time, this approach allows the study of all of the represented genes, simultaneously. These arrays likely represent about half of the chicken genome. Individual microarrays are used with pituitary or hypothalamic RNA samples isolated from animals at different stages of development or following experimental treatments. This project is aimed at identifying those genes involved in regulating growth and body composition in vertebrates.
Analysis of Global Gene Expression in the Avian Neuroendocrine System.pdf
Development of Chicken Neuroendocrine System cDNA Microarrays.pdf
 Pituitary – RedHypothalamus - Green |
 Pituitary – GreenPineal - Red |
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Bossis I, Porter TE (2001) Identification of the somatostatin receptor subtypes involved in regulation of growth hormone secretion in chickens. Mol Cell Endocrinol 182:203-213 (http://www.sciencedirect.com/science.pdf)
Bossis I, Porter TE (2003) Evaluation of glucocorticoid-induced growth hormone gene expression in embryonic pituitary cells using a novel in situ mRNA quantitation method. Mol Cell Endocrinol 201:13-23 (http://www.sciencedirect.com/science.pdf)
De Groef B, Geris KL, Manzano J, Bernal J, Millar RP, Abou-Samra AB, Porter TE, Iwasawa A, Kühn ER, Darras VM (2003) Involvement of thyrotropin-releasing hormone receptor, somatostatin receptor subtype 2 and corticotropin-releasing hormone receptor type 1 in the control of chicken thyrotropin secretion. Mol Cell Endocrinol 203:33-39 (http://www.sciencedirect.com/science.pdf)
Cogburn LA,Wang X, Carre W, Rejto L, Porter TE, Aggrey SE, Simon J (2003) Systems-wide Chicken DNA Microarrays, Gene Expression Profiling and Discovery of Functional Genes. Poultry Science 82:939-951 (http://www.poultryscience.org)
Liu L, Dean CE, Porter TE (2003) Thyroid Hormones Interact with Glucocorticoids to Affect Somatotroph Abundance in Chicken Embryonic Pituitary Cells In Vitro. Endocrinology 144:3836-3841 (http://endo.endojournals.org)
Liu L, Porter TE (2004) Endogenous thyroid hormones modulate pituitary somatotroph differentiation during chicken embryonic development. J Endocrinol 2004 180, 45–53 (http://journals.endocrinology.org)
Oubre CM, Zhang X, Clements KE, Porter TE, Berghman LR (2004) Immunohistochemical assessment of the neurosecretory cells of the chicken thymus using a novel monoclonal antibody against avian chromogranin A. Dev Comp Immunol; 28:337-345. (http://www.sciencedirect.com/)
Xiaoqin Fu and Tom E. Porter Glucocorticoid Induction of Lactotrophs and Prolactin Gene Expression in Chicken Embryonic Pituitary Cells: A Delayed Response Relative to Stimulated Growth Hormone Production Endocrinology, Mar 2004; 145: 1322 - 1330. (http://endo.endojournals.org)
Jenkins SA, Porter TE (2004) Ontogeny of the hypothalamo-pituitary-adrenocortical axis in the chicken embryo: a review. Domestic Animal Endocrinology; 26:267-275. (http://www.sciencedirect.com/science).
Bossis I, Nishimura S, Muchow M, Porter TE (2004) Pituitary expression of type I and type II glucocorticoid receptors during chicken embryonic development and their involvement in growth hormone cell differentiation. Endocrinology 145:3523-3531. The link to this reprint is http://endo.endojournals.org
Cogburn LA, Wang X, Carre W, Rejto L, Aggrey SE, Duclos M, Simon J,
Porter TE (2004) Functional genomics in chickens: Development of integrated-systems
microarrays for transcriptional profiling and discovery of regulatory
pathways. Comparative Functional Genomics 5: 253–261
(http://www3.interscience.wiley.com/cgi-bin/fulltext/108061384/PDFSTART)
Porter TE, Ellestad LE (2005) Gene expression profiling in the developing neuroendocrine system of the chick. In: Functional Avian Endocrinology, Dawson A, Sharp PJ (Eds.), Narosa Publishing House, New Dehli, pp. 45-56
Porter TE (2005) Regulation
of pituitary somatotroph differentiation by hormones of peripheral
endocrine glands Domestic Animal Endocrinology 29:52-62
Moore RW, Hargis BM, Porter TE, Caldwell DY, Oubre CM, Vandesande F,
Berghman LR (2004) Ovoinhibitor
in the chicken bursa of Fabricius: identification, isolation, and localization. Cell
Tissue Res 317:247-251
Fu X, Nishimura S, Porter TE (2004) Evidence that lactotrophs do not differentiate directly from somatotrophs during chick embryonic development. J Endocrinol 183: 417-425
Muchow M, Bossis I, Porter TE (2005) Ontogeny of pituitary thyrotrophs and regulation by endogenous thyroid hormone feedback in the chick embryo. J Endocrinol 184:407-16
Porter TE, Lopez ME, Mike R, Huberty AF (2006) The increase in prolactin-secreting cells in incubating chicken hens can be mimicked by extended treatment of pituitary cells in vitro with vasoactive intestinal polypeptide (VIP). Domestic Animal Endocrinology 30 (2006) 126–134
Porter TE, Ellestad LE, Fay A, Stewart JL, Bossis I.
Identification
of the chicken growth hormone-releasing hormone receptor (GHRH-R) mRNA
and gene: regulation of anterior pituitary GHRH-R mRNA levels by
homologous and heterologous hormones.Endocrinology. 2006 May;147(5):2535-43. Epub 2006 Feb 9.
PMID: 16469800 [PubMed - indexed for MEDLINE]
Ellestad LE, Carre W, Muchow M, Jenkins SA, Wang X, Cogburn LA, Porter TE. Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays. Physiol Genomics. 2006 May 16;25(3):414-25. Epub 2006 Feb 21.
PMID: 16493019 [PubMed - in process]
Carre W, Wang X, Porter TE, Nys Y, Tang J, Bernberg E, Morgan R, Burnside J, Aggrey SE, Simon J, Cogburn LA. Chicken
genomics resource: sequencing and annotation of 35,407 ESTs from single
and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene
index.Physiol Genomics. 2006 May 16;25(3):514-24. Epub 2006 Mar 22.
PMID: 16554550 [PubMed - in process]
Porter TE, Ghavam S, Muchow M, Bossis I, Ellestad L. Cloning of
partial cDNAs for the chicken glucocorticoid and mineralocorticoid
receptors and characterization of mRNA levels in the anterior pituitary
gland during chick embryonic development.Domest Anim Endocrinol. 2006 Jun 8; [Epub ahead of print]
PMID: 16787734 [PubMed - as supplied by publisher]
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