Research - Reproductive Development and Endocrinology of Fishes

My lab integrates molecular, endocrinological, and morphological endpoints to investigate the environment - genome interaction and its effect on development and reproduction. Through a combination of lab and field studies, my students and I study how environmental factors affect the:

• development of sex specific characteristics and
• brain - pituitary - gonadal axis regulation of reproduction in fishes

Teaching

• ANSC 212: Applied Animal Physiology
• ANSC 214: Applied Animal Physiology Laboratory
• ANSC 444: Domestic Animal Endocrinology
• ANSC 446: Physiology of Mammalian Reproduction

Publications

Representative Publications

Student co-authors are shown in bold with an asterisk

• EF Orlando, DE Bass*, LM Caltabiano*, WP Davis, LE Gray, Jr. and LJ Guillette, Jr. Altered development and reproduction in mosquitofish exposed to paper mill effluent in the Fenholloway River, Florida USA. Aquatic Toxicology 84(4): 399 - 405 (2007).
• K Watanabe, K Jensen, EF Orlando, and GT Ankley. What is Normal? A characterization of the values and variability in apical reproductive parameter data of the fathead minnow, Pimephales promelas. Comparative Biochemistry and Physiology 146(3): 348-356 (2007).
• EF Orlando, GA Binczik, ND Denslow, and LJ Guillette, Jr. Reproductive seasonality of the female Florida Gar, Lepisosteus platyrhincus. General and Comparative Endocrinology 151(3):318-324 (2007).
• J Burger, C Fossi, P McClellan-Green, and EF Orlando. Methodologies, bioindicators, and biomarkers for assessing gender-related differences in wildlife exposed to environmental chemicals. Environmental Research 104(1):135-152 (2007).
• EF Orlando and LJ Guillette, Jr. Sexual dimorphic responses in wildlife exposed to endocrine disrupting chemicals. Environmental Research 104(1):163-173(2007).
• A Kanamori, A Yamamura, S Koshiba, J-S Lee, EF Orlando, and H. Hori. Methyltestosterone efficiently induces male development in the self-fertilizing hermaphrodite fish, Kryptolebias marmoratus. Genesis: the Journal of Genetics and Development 44: 495 - 503 (2006).
• EF Orlando, Y Katsu, S Miyagawa, and T. Iguchi. Cloning and differential expression of the estrogen receptor and aromatase genes in the self-fertilizing hermaphrodite and male mangrove rivulus, Kryptolebias marmoratus. Journal of Molecular Endocrinology 37: 353 - 365 (2006).
• EF Orlando, GA Binczik, J Gates*, LE Gray Jr., M Horton*, AS Kolok, C Lambright, and LJ Guillette, Jr. Endocrine disrupting effects of cattle feedlot effluent on a sentinel species, the fathead minnow. Environmental Health Perspectives 112: 353-358 (2004).
• AM Soto, JM Calabro*, NV Prechtl, AY Yau, EF Orlando, A Daxenberger, AS Kolok, LJ Guillette, Jr., BL Bizec, IG Lange, and C Sonnenschein. Androgenic and estrogenic activity of cattle feedlot effluent receiving water bodies of eastern Nebraska, USA. Environmental Health Perspectives 112: 346-352 (2004).
• EF Orlando, GA Binczik, L Ford, P Thomas, and LJ Guillette, Jr. Reproductive seasonality of the male Florida Gar (Lepisosteus platyrhincus). General and Comparative Endocrinology 131(3) 365-371 (2003).
• EJ Durhan, C Lambright, V Wilson, BC Butterworth, DW Kuehl, EF Orlando, LJ Guillette, Jr., LE Gray, Jr., and GT Ankley. Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluent. Environmental Toxicology and Chemistry 21(9):1973-1976 (2002).
• EF Orlando, WP Davis, LJ Guillette, Jr. Aromatase activity in the ovary and brain of the mosquitofish, Gambusia holbrooki, exposed to paper mill effluent. Environmental Health Perspectives 110 (Supplement 3) 429-433 (2002).

Grants